The relationship of cell autophagy and Lovo cell migration and invasion

Abstract

Objective To investigate the influence of autophagy on migration and invasion in colon cancer cells. Methods The Lovo cells of colon cancer were classified as autophagy-enhanced group, normal cell group, and 3-Methyladenine autophagy-inhibited group.Laser canning confocal microscope was used to identify the green fluorescence particles.Beclin1 mRNA expressive level was measured with Q-PCR.Transmission electron microscope was employed to observe the autolysosome.Transwell chamber was applied to evaluate the migration and invasion of Lovo cell. Results There were more green fluorescence particles in the autophagy-enhanced group than the normal cell group and the autophagy-inhibited group.The Beclin 1 mRNA expressive level was 1.23±0.12 in the autophagy-enhanced group, while 1±0.13 in the normal cell group and 0.98±0.1 in the autophagy-inhibited group.There were the most autolysosomes in autophagy-enhanced group of all groups.The migration experiment showed the mean number of Lovo cell in the autophagy-enhanced group(138.0±16.7)were more than the normal Lovo cell group(90.7±12.9, P=0.026)and the autophagy-inhibited group(92.7±26.7, P=0.030). The invasion experiment found the mean number of Lovo cell in autophagy-enhanced group(147.0±13.0)were also more than the normal Lovo cell group(99.0±20.5, P=0.028)and the autophagy-inhibited group(95.7±25.6, P=0.021). Conclusions Autophagy enhancement could promote the migrating and invasive ability of Lovo cell.And that might be an important mechanism of invasion and metastasis in malignant tumors. Key words: Autophagy; Cell movement; Lymphocytes, tumor-infiltrating; Lovo cell