Inhibitory effects of methyl sartortuoate on invasion and migration of human colon cancer cells

Abstract

Objective To investigate the underlying mechanisms of methyl sartortuoate inhibiting invasion and migration of human colon cancer cells. Methods LoVo cells were randomly divided into control and methyl sartortuoate- treated(10, 30, and 50 μmol/L)groups. The apoptosis rate, migration, and apoptosis proteins expression changes were analyzed. Results The methyl sartortuoate in a certain concentration range inhibited the growth of LoVo and DLD-1 cells in a concentration-and time-dependent manner(P< 0.05). After treatment with methyl sartortuoate(0, 10, 30 and 50μmol/L) for 24 h, the apoptosis rate of LoVo cell was(2.09±0.97)%,(2.32±0.93)%, (11.03±0.14)% and(16.29±1.98)% respectively. After treatment with 50μmol/L methyl sartortuoate for 0, 6,12, or 24 h, the apoptosis rate was(2.67±0.97)%,(5.08±1.03)%,(5.04± 0.83)% and(13.50±1.16)%(P< 0.05) respectively. Transwell invasion assay indicated that methyl sartortuoate could inhibit invasion and migration of colon cancer Lo Vo cells. Western blotting results showed that phospho- c- Jun N- terminal kinase(JNK) and phospho- 38 expression was up- regulated in Lo Vo cells treated with methyl sartortuoate. Conclusion The results indicated that methyl sartortuoate could induce apoptosis by activating mitogen-activated protein kinase (MAPK)/JNK and P38 signaling, and further inhibit the migration and invasion of LoVo cells. Key words: Colorectal cancer; Methyl sartortuoate; Cell migration; Apoptosis