Methyl sartortuoate inhibits colorectal cancer LoVo cell growth by inducing Caspase-dependent apoptosis

Abstract

Objective To investigate methyl sartortuoate anti LoVo cells proliferative activity and the mechanism involved.Methods LoVo cells were randomly divided into control and treatment groups (10,30,50 μmol/L),methyl sartortuoate process LoVo cells apoptosis rate,cell cycle,apoptosis,protein expression changes,quantitative analysis.Results The methyl sartortuoate in a certain concentration range,concentration-dependent and time-dependent manner to inhibit the growth of LoVo cells (P ＜0.05).After methyl sartortuoate 0,10,30 and 50 μmol/L for 24 h,the LoVo cell apoptosis rate was (2.09 ±0.97)％,(2.32 ±0.93 )％,( 11.03 ±0.14)％ and ( 16.29 ± 1.98)％,50 μmol/L methyl handle processing 0,6,12,24 h.The apoptosis rates were ( 2.67 ± 0.97) ％,(5.08 ± 1.03 ) ％,(5.04 ± 0.83 ) ％and ( 13.50 ± 1.16) ％ (P ＜ 0.05 ) respectively.The cells showed typical apoptotic changes in the fluorescence microscope; LoVo cells 0,10,50 and 100 μmol/L,inhibition at the G2/M phase (9.53 ±3.97)％,(10.26±1.89)％,(11.36 ±2.56)％,(15.39±1.56)％ (P＜0.05),the proportion of gradually increased.Western blotting results showed that methyl sartortuoate processing of LoVo cells caspase-8 and caspase-3 expression was elevated B lymphocytes/leukemia-2 (bcl-2) expression decreased.Conclusion The methyl asrtortuoate can inhibit the proliferation of colon cancer LoVo cell in concentration-dependent and time-dependent manner,and induce cell apoptosis through caspase-8/caspase-3. Key words: Methyl sartortuote; Colorectal cancer; Cell proliferation; Apoptosis