Abstract

The pathological mechanism of colon cancer is very complicated. Therefore, exploring the molecular basis of the pathogenesis of colon cancer and finding a new therapeutic target has become an urgent problem to be solved in the treatment of colon cancer. ATP plays an important role in regulating the progression of tumor cells. P2 × 7 belongs to ATP ion channel receptor, which is involved in the progression of tumors. In this study, we explored the effect and molecular mechanism of ATP-mediated P2 × 7 receptor on the migration and metastasis of colon cancer cells. The results showed that ATP and BzATP significantly increased the inward current and intracellular calcium concentration of LOVO and SW480 cells, while the use of antagonists (A438079 and AZD9056) could reverse the above phenomenon. We found that ATP promoted the migration and invasion of LOVO and SW480 cells and is dose-dependent on ATP concentration (100–300 μM). Similarly, BzATP (10, 50, and 100 μM) also significantly promoted the migration and invasion of colon cancer cells in a concentration-dependent manner. While P2 × 7 receptor antagonists [A438079 (10 μM), AZD9056 (10 μM)] or P2 × 7 siRNA could significantly inhibit ATP-induced colon cancer cell migration and invasion. Moreover, in vivo experiments showed that ATP-induced activation of P2 × 7 receptor promoted the growth of tumors. Furthermore, P2 × 7 receptor activation down-regulated E-cadherin protein expression and up-regulated MMP-2 mRNA and concentration levels. Knocking down the expression of P2 × 7 receptor could significantly inhibit the increase in the expression of N-cadherin, Vimentin, Zeb1, and Snail induced by ATP. In addition, ATP time-dependently induced the activation of STAT3 via the P2 × 7 receptor, and the STAT3 pathway was required for the ATP-mediated invasion and migration. Our conclusion is that ATP-induced P2 × 7 receptor activation promotes the migration and invasion of colon cancer cells, possibly via the activation of STAT3 pathway. Therefore, the P2 × 7 receptor may be a potential target for the treatment of colon cancer.

Highlights

  • Colon cancer is one of the common digestive system tumors, which is easy to invade and metastasize, and seriously affects human physical and mental health (Machala et al, 2019)

  • We performed patch clamp and Fluo-4AM assays to evaluate the functional role of this receptor in LOVO and SW480 cells

  • We examined the effect of Effect of P2 × 7 Receptor Activation on the Invasion and Migration of Colon Cancer Cells

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Summary

Introduction

Colon cancer is one of the common digestive system tumors, which is easy to invade and metastasize, and seriously affects human physical and mental health (Machala et al, 2019). Studies have shown that ATP-induced activation of P2 × 7 receptor can promote the migration and invasion of tumor cells (Jelassi et al, 2011; Adinolfi et al, 2012). Studies have shown that reducing P2 × 7 receptor expression can inhibit the migration of breast cancer cells (Jelassi et al, 2013). The role and molecular mechanism of P2 × 7 receptor in the migration and invasion of colon cancer cells have remained unclear. The purpose of this study is to provide a new theoretical basis and data support for the treatment of colon cancer by exploring the effect and molecular mechanism of P2 × 7 receptor on the migration and invasion of colon cancer cells

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