Abstract
A comprehensive synthetic approach was employed to identify the continuous regions of T-cell recognition on the alpha-chain of Torpedo californica acetylcholine receptor (AChR). Eighteen synthetic consecutive overlapping peptides, of uniform size and overlaps, that spanned the entire extracellular part (residues 1-210) of the alpha chain were examined for their in vitro stimulation of lymph node cells from AChR-primed C57BL/6 (H-2b), C3H/He (H-2k), SWR(H-2q) and SJL (H-2s) mice. The T-cell recognition sites (T sites) in the AChR-primed mouse strains resided within six regions on the extracellular part of the alpha-chain. Three of the regions recognized by T cells coincided with regions recognized by antibodies (i.e. B cells) and one of these three regions also coincided with an alpha-neurotoxin binding region. It is noteworthy that, in addition to sites recognized by both T and B cells, the protein has at least two sites which are recognized exclusively by T cells and to which no detectable antibody responses are directed.
Published Version
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