Abstract
Stimulation of hamster lymph node cells by optimal concentrations of ZnCl 2 (10 μM) was found to be enhanced by addition of 1–25 mM LiCl to the serum-free cultures. Maximal enhancement occurred at 10 mM Li +. Similar concentrations of either KCl or NaCl did not potentiate stimulation. Addition of 1 mM CaCl 2, but not 1–25 mM MgCl 2, also potentiated Zn 2+ stimulation of lymph node cells. When the cultures were supplemented with 1 mM Ca 2+ + 10 mM Li +, a synergistic potentiation of Zn 2+ stimulation occurred. In addition, the dose response curve for Zn 2+ was shifted such that maximal stimulation occurred at 100–250 μM Zn 2+, a concentration of Zn 2+ which was toxic for the unsupplemented cultures. In Ca 2+ + Li + supplemented cultures, Zn 2+ stimulated [ 3H]thymidine incorporation to levels comparable to those obtained when hamster lymphoid cells were stimulated with lectins. In addition to Zn 2+ stimulation, Ca 2+ + Li + supplemented medium also enhanced Hg 2+ stimulation of hamster lymph node cells but did not change the dose response curve for Hg 2+. Therefore, the observed ionic effects on Zn 2+ stimulation of lymphocytes were unique to this mitogen, when compared to either Hg 2+ stimulation or previously reported lectin stimulation of hamster lymphoid cells.
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