The protective effects of hydrogen inhalation on renal injury in severe septic mice

Abstract

Objective To investigate the protective effects of hydrogen gas (H2) inhalation on acute renal injury in severe septic mice,and the associated mechanism.Methods Sepsis model was made by cecal ligation and puncture (CLP) operation in mice.A total of 24 mice were randomly (random number)divided into four groups:sham operation group (Sham),sham operation with H2 inhalation group (Sham +H2),sepsis group (Sepsis),and sepsis with H2 inhalation group (Sepsis + H2).The procedure for inhalation of 2％ H2 for 1 h was initiated at 1 h after CLP and 6 h after sham operation,respectively.The pathological changes and apoptosis of kidney,the blood creatinine (Cr) and blood urea nitrogen (BUN)concentration,the activities of superoxide dimutase (SOD) and catalase (CAT) in serum and kidney,as well as the levels of 8-iso-prostaglandin F2α (8-iso-PGF2α) and high mobility group box 1 (HMGB1) in serum and kidney were observed at 24 h after operation.Results H2 inhalation could significantly alleviate the renal injury of septic mice,reduce the renal cell apoptosis,increase the activities of SOD and CAT,as well as decrease the levels of 8-iso-PGF2α and HMGB1 in serum and kidney (P ＜ 0.05).Conclusions H2 inhalation can alleviate acute renal injury in septic mice,resulting from the increased activities of antioxidant enzymes and the reduced levels of oxidative products and inflammatory cytokines. Key words: Hydrogen ; Sepsis ; Acute renal injury ; Antioxidant enzyme ; Inflammatory cytokine