Abstract
Objective To investigate the protective effect of hydrogen-rich saline on lung injury associated with severe acute pancreatitis and its impact on P38MAPK and NF-κB expressions. Methods Fifty-four male Wistar rats were randomly (random number) divided into three groups: (1) hydrogen-rich saline treatment group (HRS group, n=18 ) , in which the rats were treated with hydrogen-rich saline (6 mL/kg) administered intravenously via tail vein and HRS (20 mL/kg) administered subcutaneously at 5 min after successful modeling. (2) Severe acute pancreatitis model group (SAP group, n=18) , in which rats received equivalent volume of normal saline instead of hydrogen-rich saline both intravenously and subcutaneously as in HRS group. (3) Sham operation group (SO group, n=18) , in which rats were treated with sham surgery, and received equivalent volume of normal saline as in SAP group. The model of severe acute pancreatitis (SAP) was made by retrograde injection of 5% sodium taurocholate (1 mL/kg) into cholepancreatic duct. All rats were sacrificed at 3 h, 12 h, and 24 h separately after the operation (n=6 at a time). The levels of serum amylase, lipase were measured. The ratio of wet and dry lung tissues was measured. The histopathological changes of lung tissues were observed under optic microscope. The expressions of P38MAPK, p-P38MAPK and NF-κB were measured by using immunohistochemistry method. Results Compared with SAP group, there were no significant differences in levels of serum amylase [12 h (5 306.7±909) vs. (5 435.0±441.2) ]and lipase [12 h (1 897.8±149.4) vs. (1 917.9±106.8) ]in HRS group (P >0.05) , but there were significant differences in the ratio of wet and dry lung tissues [12 h (3.12±0.58) vs. (1.87±0.25) ]and histopathology scores [12 h (2.14±0.38) vs. (3.58±0.32) ] (P <0.05). There was no significant difference in expression of P38MAPK in lung tissues among three groups at 12 h. Compared with SO group, the expressions of p-P38MAPK and NF-κB were significant increased in SAP group at 12 h, however, they were lower significantly in HRS group than those in SAP group. Conclusions Hydrogen-rich saline has a protective effect on lung injury associated with severe acute pancreatitis, and its mechanism may be likely related to the antioxidant effect and inhibiting the activation of P38MAPK and NF-κB. Key words: Hydrogen-rich saline; Pancreatitis; Lung injury; Protective effect; P38MAPK; NF-κB
Published Version
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