The process of immobilizing enzyme of glucose sensor based on diamond film

Abstract

Considering the poor adhesion of electrode to substrate, diamond film as a new kind of substrate material was used to fabricate a glucose sensor. Particularly, the immobilizing enzyme was investigated in detail. SEM and XPS were chosen to identify whether organic functional groups were grafted to electrode surface or not. The response characteristics of a diamond film glucose sensor show that this glucose sensor has good properties in the linear range 0.5–11.4 mM l -1, sensitivity 4.0 nA mM -1 mm -2 and peak reaction speed 2.5 μA. The glucose sensor based on diamond film was a novel microchip glucose sensor with good potential.