Abstract
A method for the preparation of tRNA from Escherichia coli is described. A crude tRNA is prepared by phenol and water treatment of cells without the need of centrifugation for the separation of an aqueous phase from a phenolic phase containing cellular debris. Dimethyl sulfoxide and NaCl are used for the purification of the tRNA in good yield without a requirement for column chromatography. Amino acid acceptance assays are used to quantitate the purification and to characterize the preparations.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
