Abstract
A method for the preparation of rat-liver sRNA is described, where contamination with other RNA's and DNA is minimized. Sephadex G-200 chromatography was used to free the RNA of most high- and low-molecular-weight contaminants. Ratliver sRNA purified by this method has approximately twice the serine acceptor activity as yeast sRNA, using a rat-liver enzyme preparation. The method is suitable for the preparation of gram quantities of sRNA.
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