Abstract
A large number of microhaplotype markers have been investigated for various forensic purposes in recent years. In the present study, we have analysed population genetic data and sequencing performance of the Ion AmpliSeq™ MH-74 plex microhaplotype research panel. A total of 178 samples from 3 major biogeographic regions including 54 Africans, 61 Iraqis from the Middle East and 63 Germans were genotyped using this panel on the Ion S5™ MPS platform (Thermo Fisher Scientific). Then we obtained the allele frequencies, the effective number of alleles (Ae), and the forensic efficiency parameters of the 74 MHs. Finally, STRUCTURE analysis was used to evaluate and compare the biostatistical efficiency of the 74 MHs in the 3 major biogeographic regions. All results demonstrated that the Ion AmpliSeq™ MH-74 plex microhaplotype research panel is characterized by a reliable sequencing performance.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
More From: Forensic Science International: Genetics Supplement Series
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.