The performance of hypochlorous acid modified Ag nanoparticle-based assay in the determination of total antioxidant capacity of Boswellia Serrata and Aronia

Abstract

In this study, the SNPAC method was developed by using hypochlorous (ClO−) at pH 9.3. Polyvinylpyrrolidone (PVP) was used as a stabilizer. The existence of HOCl enables to formation stronger and more stable yellow emission which gives sensitivity. As a result, lower LOD and LOQ values were achieved by the AgNP–HOCl method. The obtaining lower values was attributed to the reoxidation of silver atoms with ClO− ions in the presence of PVP. The newly modified method was applied to the total antioxidant capacity (TAC) measurement of Boswellia Serrata, Aronia Melanocarpa tea, and juice. The results were compared with the results obtained by application of CUPRAC (Cupric ion Reducing Antioxidant Capacity) and FRAP (Ferric Reducing Antioxidant Power) assays. The TAC values of Boswellia Serrata, Aronia Melanocarpa tea, and juice were found as 6.18, 137.98, and 12.08 mmol TEAC/kg, respectively.