Abstract
The pattern of DNA replication in cells of Chinese hamster has been studied using graincounts over individual chromosomes following labelling with 3H-thymidine and autoradiography. Cells which had undergone the same proportion of the DNA synthesis period in the presence of label were identified by the proportion of grains over sex chromosomes. It was then shown that, within tissues, cells selected in this way were statistically homogeneous with respect to the distribution of grains over the autosomes. These distributions did not differ significantly between cornea, thymus, spleen and bone marrow. Evidence is presented that the most rapidly dividing of these tissues is thymus and that in one individual its cell cycle time averaged about 8 hr. Evidence is also given that intraperitoneally injected 3H-thymidine is available to the chromosomes of spleen, marrow, thymus and cornea in the ratio of about 4:2:1:1.
Published Version
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