Abstract
In thin-layer chromatography (TLC), there are only three degrees of freedom in which the chromatographic results can be seriously influenced. They are (1) the polarity of the stationary phase in partition chromatography or its activity in adsorption chromatography; (2) the selection of the mobile phase; and (3) the composition of the vapour phase in contact with the layer. Although all three factors can change a separation, the most important for method development is the mobile phase composition.
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