The Mechanism of Nano-Particles Intervening Invasion and Metastasis of Lymphoma Based on Autophagy Targeted with miR-36b and Orienteering Analysis on Apoptosis Gene

Abstract

Whether the expression of gene P53 related with autophagy and apoptosis and action was regulated by miR-36b was discussed in our study. And the action of orienteering nano-particles on intervening invasion and metastasis of lymphoma was analyzed. The normal lymphoid tissue collected from the patients with simple lymphatic hyperplasia was set as control. The lymphoma samples from patients with early indolent lymphoma were collected. The level of mRNA in miR-36b and P53 was detected by PCR. The level of P53 protein and level of mRNA in miR-36b and P53 among normal lymphoid cell, cell strain of low metastatic lymphoma and cell strain of high metastatic lymphoma was compared. They were divided into four groups: miR-NC group, orienteering nano-particles’ group, siRNA-NC group and siRNA-P53 group. The cell proliferative capacity was detected by FCM. The quantity of cell invasion and metastasis was detected by transwell. The expression quantity of P53 mRNA in lymphoma tissue was increased obviously compared with control group. The expression of miR-36b was lower while the expression of P53 was higher along with the later staging of TNM. And the express was related with the staging of TNM. The expression quantity of P53 mRNA in lymphoma cell was higher in normal cell notably. But expression quantity of miR-36b in lymphoma cell was lower in normal cell notably. The decreased of expression of miR-36b and increased of expression of P53 was related with enhancing the ability of invasion and metastasis of lymphoma cells.