Abstract
1. DNA was isolated from RecA and wild-type extracts, prepared by ultrasonication of cell suspensions of Escherichia coli and analysed on neutral sucrose gradients. DNA from the RecA strain showed two peaks, one corresponding to 6 S and the other to 12 S, while DNA from the wild-type strain was found to be only of the 12-S type. 2. The 12-S DNA from the RecA mutant, as well as from the wild-type strain, interferes with the ATP-dependent nuclease activity measurement, suggesting that it competes with the added 32P-labelled DNA as a substrate. However, nearly 30 % of the RecA DNA is present in the 6-S fraction and this DNA does not affect the measurement.
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