Abstract
The fatty acid composition of Dictyostelium discoideum has been modified by growing the axenic strain, Ax-2, in media containing long chain polyenoic fatty acids. Large amounts of linoleic and linolenic acids are incorporated into the cellular lipids and further desaturated to two unusual fatty acids, 5,9,12-octadecatrienoic acid and 5,9,12,15-octadecatetraenoic acid, respectively. Arachidonic acid is also extensively incorporated but not further desaturated. D. discoideum normally contains none of the above polyenoic fatty acids, and the amount incorporated depends upon the concentration of the fatty acid in the growth media. The cells containing large quantities of polyenoic fatty acid grow normally but exhibit impaired differentiation when removed from the growth medium. The incorporation of smaller quantities of the fatty acid has no adverse effect on differentiation. Cells grown in the presence of saturated or monoenoic fatty acids exhibit, at the most, only slight changes in the fatty acid composition of the cellular lipid and both grow and differentiate normally.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
