Abstract
Fatty acid α-oxidation in cucumber ( Cucumis sativus) involves enzymatic conversion of long-chain C n -fatty acids to the C ( n−1) -aldehyde and CO 2. However, the mechanism of this process is not well understood. In this study, the α-oxidation of the fatty acid analogues tetradecylthioacetic acid (TTA) and tetradecylthiopropionic acid (TTP) with a sulphur atom substituting the methylene group in positions 3 and 4, respectively, was investigated and compared to palmitic acid. Both [1- 14C]TTA and [1- 14C]TTP could be α-oxidised in the cucumber subcellular 150 000× g max fraction. [1- 14C]TTP was an even better substrate compared to the natural palmitic acid, while [1- 14C]TTA was α-oxidised to a lower extent. [2- 14C]TTA revealed no 14CO 2, indicating that only one cycle of α-oxidation occurred. TTA was an inhibitor of the palmitic acid α-oxidation, and the inhibitory effects were examined.
Published Version
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