Abstract
The isolation of a new, third phospholipase A 2 from Agkistrodon halys blomhoffii is described. On the basis of a p I value of 6.9, it is termed a neutral phospholipase A 2. It was characterized as to its amino acid content, activity towards phosphatidylcholine, heat stability, and hemolytic behavior on human erythrocytes. A comparison of these characteristics with those of the acidic and basic phosphplipases A 2 established the uniqueness of the neutral enzyme. Two particularly important observations were concerned with the complete stability of the three phospholipases on heating at 100°C at pH 6.0 in the presence of 10 mM Ca 2+, but variable stability in the absence of Ca 2+, and the significant lack of hemolytic activity by the acidic (p I 4.9) phospholipase A 2 as compared to the neutral (p I 6.9) and basic (p I 8.7) enzyme which produced extensive hemolysis of human erythrocytes. Other facets of the characteristics of these phospholipases are discussed.
Published Version
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