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Abstract
1. Ribosomal RNA is isolated from 87-S Euglena gracilis ribosomes under a variety of salt and temperature conditions in order to study the integrity of the 25-S RNA molecule. 2. Both polyacrylamide gel electrophoresis and zone velocity sedimentation in sucrose gradients are used to monitor the degradation process. 3. In line with earlier reports it was found that Na + (0.2 M) in the isolation milieu stabilizes the 25-S RNA. 4. The isolated 25-S RNA is readily degraded at 37–45° independent of the ionic conditions used during the isolation procedure. 5. The 20-S RNA is stable under identical conditions. 6. Under mild degradative conditions a novel 23-S RNA is formed. We postulate it to be a distinct degradation product of the 25-S RNA. 7. This 23-S RNA is clearly resolved in the polyacrylamide gel but not necessarily in the sucrose gradient. 8. The 25-S RNA molecule seems to be partly cleaved at selected loci in situ; depending on the isolation conditions the 25-S RNA molecule may undergo further degradation during the deproteinization procedure.
Published Version
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