The influence of the operation conditions and the extraction techniques on the yield, kinetics and the composition of ethanol extracts of Hieracium pilosella L.

Abstract

The optimal operational extraction conditions (50 % v/v ethanol, solvomodule l:15 m/v, temperature: the solvent boiling temperature) were determined by investigating the influence of the ethanol concentration (30 - 95 % v/v), solvomodule (1:10 - 1:25 m/v) and the temperature of the maceration extraction on the yield and kinetics of total extraction matter, chlorogenic acid, umbelliferone and apigenin-7-O-glucoside from Hieracium pilosella L. Based on the results of Soxhlet and Tillepape extraction kinetics, the investigations of the total extraction matter and the components under the optimal maceration operation conditions it was found that the highest yield of the extraction matter from the dry plant material (43.0 g/100 g of the dry plant material, i.e. 97.7% of the extractive matter content in the plant material), chlorogenic acid (20.30 g/100 g of dry plant material, i.e. 94 % of the chlorogenic acid content in the plant material), umbelliferone (0.30 g/100 g of the dry plant material, i.e. 96.7% of umbelliferone content in the plant material, and apigenin-7-O-glucoside (0.24 g/100 g of the dry plant material, i.e. 96 % of the apigenin-7-O-glucoside content in the plant material) was obtained by using a Soxhlet extraction method. The contents of chlorogenic acid, umbelliferone and apigenin-7-O-glucoside in the extracts were determined by HPLC method. Chlorogenic acid is the component with the highest share in all the extracts. (It was found) The high content of chlorogenic acid in the investigated plant material (21.60 %) and the high level of its extraction from the plant material (75-94**%) were found under the defined optimal maceration conditions, Soxhlet and Tillepape extraction.