The Influence of Cu and Co Nanoparticles on Growth Characteristics and Biochemical Structure of Mentha Longifolia in Vitro

T.E Talankova-Sereda,E A Shkopinskij,N.I Kucenko,P.G Dulnev,A.V Kovalyova,A.I Ustinov,K.V Liapina

doi:10.13189/nn.2016.040201

Abstract

The aim of the given work was to investigate the influence of copper and cobalt nanoparticles on clonal microreproduction of Mentha longifolia plants received by method of isolated cells and tissues culture. Copper and cobalt nanoparticles were used in the form of colloidal solutions of high viscosity (gels). As object of research were Mentha longifoliа plants. During experiments the optimum concentration of copper (0,5 mg/l) and cobalt (0,8 mg/l) nanoparticles colloidal solutions have been defined. These solutions were added in Murasige and Skug nutrient medium and have led to increase of microplant height and growth index on 45-48,4 %, the quantity of internodes on 29,4-33,9 % and quantity of shoots on 55,6-66,2 %, reproduction coefficient on 30-40 %.

Highlights

Despite the big achievements in biotechnology of plants, there are certain difficulties in definition of necessary components and concentration of macro, microelements and growth regulators in nutrient medium for various kinds and grades of plants, purposely to receive the greater quantities of plants-regenerants.It is known, that microelements activate definite enzymatic systems by direct participation in structure of enzymes molecules or by their activation
Thereupon, the investigation of copper and cobalt nanoparticles influence on Mentha longifolia explants growth characteristics depending on nanoparticles concentration at clonal reproduction in vitro and changes of basic biochemical indices of essential oils is actual
The properties of received colloidal solutions were investigated by means of device Malvern Zetasizer Nano S (Malvern Instruments Ltd., the Great Britain) Ver. 6.20 in which particles size is defined on their moving with use of Einstein-Stoks relation

Summary

Introduction

Despite the big achievements in biotechnology of plants, there are certain difficulties in definition of necessary components and concentration of macro-, microelements and growth regulators in nutrient medium for various kinds and grades of plants, purposely to receive the greater quantities of plants-regenerants.It is known, that microelements activate definite enzymatic systems by direct participation in structure of enzymes molecules or by their activation. Thereupon, the investigation of copper and cobalt nanoparticles influence on Mentha longifolia explants growth characteristics depending on nanoparticles concentration at clonal reproduction (cultivation) in vitro and changes of basic biochemical indices of essential oils is actual.

Results

Conclusion