Abstract
Two-dimensional and three-dimensional crystalline arrays of turnip rosette virus (TRosV) have been formed using the negative-staining carbon-film technique for electron microscopy. Three types of packing arrangements of the virus within the lattice arrays have been observed, hexagonal, square, and skewed. Analysis of the electron micrographs, by optical diffraction, showed considerable differences in the packing of the particles compared with other viruses prepared by the same technique. It was found that there was a predominant close hexagonal packing over a wide range of pH values for the first negative stain in which the crystalline arrays were formed on different surface materials. The individual particles seen in the arrays were observed to have a central area of varying contrast. Flotation of the crystalline arrays at the liquid/air surface of a second negative stain at high pH resulted in the gradual disruption of the individual capsids within the lattice.
Published Version
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