Abstract
A simple technique has been developed for the preparation of highly concentrated or crystalline suspensions of viruses in the presence of negative stains. Several icosahedral and filamentous viruses have been studied in concentrations from 1.0–20 mg/ml. The technique has several advantages over conventional negative staining procedures by forming two-dimensional and three-dimensional crystalline arrays of highly concentrated viruses. The packing arrangements of the virus in the crystalline arrays can be varied according to the type of negative stain and pH used during the preparation. High resolution electron micrographs of material prepared by the method have been analysed by optical diffraction procedures. The relative thickness of the carbon supporting film in relation to the physical dimensions of viruses is considerably improved.
Published Version
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