The importance of experimental milieu in regulation of macrophage activation

Abstract

Event Abstract Back to Event The importance of experimental milieu in regulation of macrophage activation Michaela Pekarova1*, Antonin Lojek1, Hana Martiskova1, Anna Klinke2, Tanja K. Rudolph2, Barbora Svobodova1, Michaela Maceckova1, Radek Kuchta3, Jaroslav Kadlec3, Zdenka Kuchtova3 and Lukas Kubala1 1 Institute of Biophysics, AS CR v.v.i., Department of Free Radical Pathophysiology, Czechia 2 University Hospital of Cologne, Germany 3 Brno University of Technology, Faculty of Electrical Engineering and Communication, Czechia Background: Macrophages are important source of cytokines and other compounds, which recruit additional cells to sites of infection or tissue injury. Importantly, their functions might be directly controlled by the composition of milieu in their immediate vicinity. Therefore, the analysis of their function under different in vitro as well as “simulated” in vivo condition is of particular interest. Materials and methods: Mice peritoneal macrophages were stimulated with different activators (e.g. LPS, IFN-γ, TNF-α and interleukins or their combination) under different experimental conditions (in vitro and “simulated” in vivo conditions). Consequently, the physiological functions of macrophages were assessed using different luminometric, spectrophotometric, molecular, and immunohistochemical methods. Results: Our data demonstrate that there exists crucial difference in activation of macrophages under classical in vitro and “simulated” in vivo conditions. These abnormalities are accompanied by significant changes in inflammatory response of macrophages as well as in activation of intracellular signaling pathways. Conclusions: The activation of mice peritoneal macrophages is dependent on the composition of milieu in their immediate vicinity and the “classically used” in vitro systems seems to be not suitable for correct analysis of their functions. Acknowledgements This work was supported by the Academy of Sciences of the Czech Republic (M200041208, AV0Z50040702), and by The Ministry of Education, Youth and Sports (CZ.1.07/2.3.00/30.0030). Keywords: Macrophages, experimental milieu, activation, lipopolysacchride, Interferon-gamma Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Innate immunity Citation: Pekarova M, Lojek A, Martiskova H, Klinke A, Rudolph TK, Svobodova B, Maceckova M, Kuchta R, Kadlec J, Kuchtova Z and Kubala L (2013). The importance of experimental milieu in regulation of macrophage activation. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00366 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 15 Mar 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. Michaela Pekarova, Institute of Biophysics, AS CR v.v.i., Department of Free Radical Pathophysiology, Brno, 61265, Czechia, pekarovam@ibp.cz Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Michaela Pekarova Antonin Lojek Hana Martiskova Anna Klinke Tanja K Rudolph Barbora Svobodova Michaela Maceckova Radek Kuchta Jaroslav Kadlec Zdenka Kuchtova Lukas Kubala Google Michaela Pekarova Antonin Lojek Hana Martiskova Anna Klinke Tanja K Rudolph Barbora Svobodova Michaela Maceckova Radek Kuchta Jaroslav Kadlec Zdenka Kuchtova Lukas Kubala Google Scholar Michaela Pekarova Antonin Lojek Hana Martiskova Anna Klinke Tanja K Rudolph Barbora Svobodova Michaela Maceckova Radek Kuchta Jaroslav Kadlec Zdenka Kuchtova Lukas Kubala PubMed Michaela Pekarova Antonin Lojek Hana Martiskova Anna Klinke Tanja K Rudolph Barbora Svobodova Michaela Maceckova Radek Kuchta Jaroslav Kadlec Zdenka Kuchtova Lukas Kubala Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.