Chimeric protein of IL-2 linked to light chain of anti-IL-2 mAb S4B6 that structurally mimics IL-2/S4B6 immunocomplexes: design and production

Abstract

Event Abstract Back to Event Chimeric protein of IL-2 linked to light chain of anti-IL-2 mAb S4B6 that structurally mimics IL-2/S4B6 immunocomplexes: design and production Barbora Dvorakova1*, Jakub Tomala1, Jirina Kovarova1, Martina Kabesova1, Petra Votavova1, Helena Chmelova1, Blanka Rihova1 and Marek Kovar1 1 Institute of Microbiology ASCR, Czechia IL-2/anti-IL-2 mAb immunocomplexes were reported to have significantly higher biological activity than free IL-2 in vivo. Depending on the mAb used they show different selectivity of their stimulatory activity. However, possible dissociation of IL-2 from mAb or excess of either substance in prepared immunocomplexes are general disadvantage. In order to overcome such problems, we designed and produced protein chimera consisting of IL-2 linked to light chain of anti-IL-2 mAb S4B6 through flexible oligopeptide spacer (Gly4Ser)3. The intramolecular interaction of IL-2 and mAb part in our protein chimera is similar to that in immunocomplexes of IL-2 and anti-IL-2 mAb S4B6 (henceforth IL-2/S4B6), but dissociation of IL-2 too far away from mAb is prevented by covalent attachment to S4B6 mAb. We showed the produced protein chimera has predicted molecular weight slightly higher than IL-2/S4B6, contains both IL-2 and S4B6 mAb in one molecule and IL-2 interacts with binding site of S4B6 mAb in cis. Thus, we conclude that IL-2-S4B6 protein chimera mimics IL-2/S4B6 immunocomplexes in terms of structure and cytokine-mAb interactions. Acknowledgements This work was supported by Czech Science Foundation grant P301/11/0325, by the Ministry of Education, Youth and Sports grant CZ.1.07/2.3.00/20.0055 and by Institutional Research Concept RVO 61388971. Keywords: IL-2, immunocomplexes, protein chimera, anti-IL-2 mAb, S4B6 mAb, expression vectors Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Translational immunology and immune intervention Citation: Dvorakova B, Tomala J, Kovarova J, Kabesova M, Votavova P, Chmelova H, Rihova B and Kovar M (2013). Chimeric protein of IL-2 linked to light chain of anti-IL-2 mAb S4B6 that structurally mimics IL-2/S4B6 immunocomplexes: design and production. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00123 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 10 Mar 2013; Published Online: 22 Aug 2013. * Correspondence: Ms. Barbora Dvorakova, Institute of Microbiology ASCR, Prague, Czechia, Belandy.224@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Barbora Dvorakova Jakub Tomala Jirina Kovarova Martina Kabesova Petra Votavova Helena Chmelova Blanka Rihova Marek Kovar Google Barbora Dvorakova Jakub Tomala Jirina Kovarova Martina Kabesova Petra Votavova Helena Chmelova Blanka Rihova Marek Kovar Google Scholar Barbora Dvorakova Jakub Tomala Jirina Kovarova Martina Kabesova Petra Votavova Helena Chmelova Blanka Rihova Marek Kovar PubMed Barbora Dvorakova Jakub Tomala Jirina Kovarova Martina Kabesova Petra Votavova Helena Chmelova Blanka Rihova Marek Kovar Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.