Role of Slc11a1 gene in macrophage activation during BCG infection

Abstract

Event Abstract Back to Event Role of Slc11a1 gene in macrophage activation during BCG infection Priscilia Aguilar-Ramirez1, Wafa Cabrera1, José R. Jensen1, Dunia Rodriguez2, Luciana C. Leite2, Orlando Ribeiro1, Marcelo De Franco1, Olga M. Ibañez1 and Nancy Starobinas1* 1 Instituto Butantan, Brazil 2 Instituto Butantan, Brazil The Slc11a1 gene regulates resistance against S. enterica Typhimurium, L. donovani and M. tuberculosis. AIRmax and AIRmin mouse sublines, homozygous for Slc11a1 R and S alleles (AIRmaxRR, AIRmaxSS, AIRminRR and AIRminSS) were produced in our Lab to study the effect of this gene in the inflammation background. The aim of this work is to evaluate the effect of this gene in peritoneal macrophage (MΦ) activation during M. bovis BCG infection. Mice were inoculated ip with BCG (14 days) or PBS (control). The peritoneal cells were collected and placed in culture, the adherent MΦ stimulated or not with LPS were used in all experiments. Spleens were homogenized and cultured in MB7H10 medium and the bacterial colonies were counted. Production of NO, H2O2 and cytokine were determined in culture supernatants. In BCG infection only AIRmaxRR mice were capable of controlling bacterial proliferation, which was accompanied with high levels of IL-1β, IL-12, TNFalfa and IL-6 produced by activated macrophages. On the other hand, susceptible AIRminSS mice produced higher amounts of NO, H2O2 and IL-10 suggesting that both inflammatory background and Slc11a1 alleles interfere on resistance to BCG infection. Thus, we conclude that the high inflammatory response associated to Slc11a1 R alleles (in AIRmaxRR mice) are mechanisms for efficient bacterial killing, through production of cytokines by MΦ which activates the immune response. Financial support: CNPq, FAPESP Keywords: macrophage, mice genetically selected, Cytokines, TNF alfa, NO Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Innate immunity Citation: Aguilar-Ramirez P, Cabrera W, Jensen JR, Rodriguez D, Leite LC, Ribeiro O, De Franco M, Ibañez OM and Starobinas N (2013). Role of Slc11a1 gene in macrophage activation during BCG infection. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00296 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 12 Mar 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. Nancy Starobinas, Instituto Butantan, São Paulo, Brazil, nancystar@butantan.gov.br Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Priscilia Aguilar-Ramirez Wafa Cabrera José R Jensen Dunia Rodriguez Luciana C Leite Orlando Ribeiro Marcelo De Franco Olga M Ibañez Nancy Starobinas Google Priscilia Aguilar-Ramirez Wafa Cabrera José R Jensen Dunia Rodriguez Luciana C Leite Orlando Ribeiro Marcelo De Franco Olga M Ibañez Nancy Starobinas Google Scholar Priscilia Aguilar-Ramirez Wafa Cabrera José R Jensen Dunia Rodriguez Luciana C Leite Orlando Ribeiro Marcelo De Franco Olga M Ibañez Nancy Starobinas PubMed Priscilia Aguilar-Ramirez Wafa Cabrera José R Jensen Dunia Rodriguez Luciana C Leite Orlando Ribeiro Marcelo De Franco Olga M Ibañez Nancy Starobinas Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.