Abstract
The untranscribed DNA sequences of the intergenic spacer regions (ISR) in the groESL were analyzed to resolve the ambiguity within phylogenetically close GRAS and non-GRAS species. The sequencing results of amplified polymerase chain reaction (PCR) products using groESL-ISR-specific primers accurately distinguished L. plantarum and L. pentosus, which were not distinguished by 16 S rRNA sequences. Furthermore, the 20 selected major probiotics species were divided into several groups according to the length (22–91 bp) and homology (65–99%) of their ISR sequences, and this discrimination was consistent with core-genome-based differentiation. Therefore, ISR sequence-based species identification showed more accurate results than 16 S rRNA sequence-based identification and represented genome-based speciation.
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