Abstract

After the injection of estrone- 3H-sulfate- 35S into a rabbit, a double conjugate containing the same 3H 35S ratio was obtained from the urine. Removal of sulfate from this material yielded a monoconjugate which crystallized to constant specific activity with estradiol-17α-N-acetyl-glucosaminide. Experiments in vitro with rabbit liver microsomes confirmed this evidence that the steroid N-acetylglucosaminyl transferase can use 17α-estradiol-3-sulfate as a substrate. The transfer of N-acetylglucosamine to 15α-hydroxyestrone-3-sulfate was also demonstrated in vitro .

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