Abstract
Objective To explore the functions of PTRF in non-small cell lung cancer. Methods Two short hairpin RNA (shRNA) and one control shRNA were constructed. And then blank, control, shRNA-A and shRNA-B were transfected in A549 and mRNA and protein levels were detected using reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blotting. Wound healing and invasion assays were done to detect the capability of migration and invasion in A549 cell lines. Results mRNA and protein levels of PTRF in cancer tissues were significantly lower than those in adjacent cancer tissues(P=0.003, P=0.016). mRNA levels of PTRF in shRNA-A and shRNA-B were lower than those in controls(P=0.009, P=0.012). Protein levels of PTRF in shRNA-A and shRNA-B were lower than those in controls(P=0.002, P=0.005). Higher levels of migration and invasion were observed in A549 cell lines after transfection with shRNA-A and shRNA-B(P=0.018, P=0.029 and P=0.024, P=0.038). Conclusion PTRF was decreased and promoted migration in lung cancer. Key words: Polymerase I and transcript release factor; Non-small cell lung cancer; Migration; Small interfering RNA
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