The experimental study on alteration of intestinal mucosal barrier function in rats with intestinal obstruction

Abstract

Objective To investigate the dynamic changes of the intestinal barrier function in rats after in-testinal obstruction .Methods The rats model of intestinal obstruction was established and samples of tissue ,mesen-teric lymph nodes and serum were collected at different intervals .The serum diamine oxidase （ DAO） and intestinal fatty acid binding protein（IFABP） were determined,the gut tissue was examined microscopically and the incidence of bacterial translocation was identified by mesenteric lymph nodes .Results The serum concentration of IFABP began to increase at 12h,and increased gradually with the passage of time of intestinal obstruction .In addition,it was noted that there was a significant positive correlation between the serum concentration of IFABP and the degree of intestinal mucosa change（r=0.841,P〈0.01）.DAO activity increased after 24h of intestinal obstruction,up to the peak at 48h and declined by followed time .The mesenteric lymph node bacterial translocation began to arise after 48h,which was significantly higher than the sham-operated group（P〈0.01）.Conclusion The intestinal barrier was damaged dur-ing early stage of intestinal obstruction .The occurrence of bacterial translocation is obviously slower than the change of serum biological indicators .It was found that serum IFABP can reflect the degree of intestinal barrier injury , which contributes to judging the course of disease and preventing the complications after intestinal obstruction . Key words: Intestinal obstruction; Intestinal fatty acid binding protein; Diamine oxidase; Bacterial translocation