THE EXPERIENCE OF OBTAINING A CELL CULTURE OF BREAST CARCINOMA FROM A TUMOR LUMINAL B SUBTYPE

Abstract

The paper describes a case of obtaining a primary culture of breast carcinoma from tumor cells of the luminal B subtype. The aim of the study. Assess the cell morphology and changes in the receptor apparatus when obtaining a primary cell culture and further maintaining it during five passages Methodology. The paper describes the case of obtaining a primary culture of breast cancer. The level of expression for the following markers was determined: pancytokeratin, vimentin, the level of proliferative activity of Ki-67. Results. The data on the polyformism of cells in culture are presented, the levels of cell proliferation are estimated, the epithelial nature of the cells is determined during five passages. At the fourth passage, signs of an epithelial-mesenchymal transition were noted. The level of Ki-67 proliferative activity differed at different passages and was higher or lower than the initially established level in the primary material. The study of the primary culture and the first passages of the cell line is important for creating a further relevant model for the tumor in vivo. Conclusion. It is necessary that such a model be as close as possible in terms of cellular composition and receptor apparatus to the primary tumor.