The Enzyme-Like Property and Photocatalytic Effect on α, α-Diphenyl-β-Picrylhydrazyl (DPPH) of CuPt Nanocomposite

Tao Wen,Jie Meng,Doudou Yan,Haiyan Xu,Jian Liu

doi:10.3390/catal9100813

Abstract

With co-reduction method, a new nanocomposite consisting of Cu and Pt (CuPt) was prepared in household. The morphology of CuPt alloy was characterized by scanning electron microscope (SEM) and transmission electron microscope (TEM). The diameter of CuPt was ca. 125 nm measured by dynamic light scattering (DLS). The atom ratio of Cu to Pt was determined to be about 1.2 by energy-dispersive X-ray (EDX). Enzyme-like activities of CuPt, including peroxidase (POD)-like and ascorbic acid oxidase (AAO)-like activities were examined with UV-Vis-NIR spectrometer. The CuPt was found to interact with α, α-diphenyl-β-picrylhydrazyl (DPPH) in the presence or absence of AA. With irradiation by light emitting diode (LED) light, the photocatalysis effect of CuPt on DPPH was investigated. With the addition of histidine, it was proved that singlet oxygen had an important role in the interaction involving CuPt. The new nanocomposite and the properties suggest various potentials of application.

Highlights

Certain achievements have been made in the metal nanomaterials as heterogeneous catalysts, and this has led to various applications [1]
Due to the fact that 2,32,3-diaminophenazine has an extinction peak at 450 nm, which is produced by POD-catalyzed oxidation diaminophenazine has an extinction peak at 450 nm, which is produced by POD-catalyzed oxidation of OPD in the presence of hydrogen peroxide [16], we investigated the change of extinction at 450 nm of OPD in the presence of hydrogen peroxide [16], we investigated the change of extinction at 450 nm to determine the oxidation degree of OPD
The extinction decreased significantly in the presence of histidine, which suggested the singlet oxygen play a major role in the reaction of ascorbic acid (AA) and Cu and Pt (CuPt) (Figure 4D). These results demonstrated that CuPt can reduce the antioxidant activity of AA

Summary

Introduction

Certain achievements have been made in the metal nanomaterials as heterogeneous catalysts, and this has led to various applications [1]. The enzyme-like activity and antioxidant activities of metal nanomaterials have attracted intensive research interests in recent years. To date, nanomaterials such as ferromagnetic nanoparticles, FeS, CuS, ZnS, and noble metals have been reported to hold peroxidase (POD)-like, oxidase-like, catalase-like, superoxide dismutase (SOD)-like, or ascorbic acid oxidase (AAO)-like properties [2,3], and have shown promising potentials in several medical and biological detection systems [4]. Nanoparticles containing Cu and Pt element have been used in various applications: Cu-Pt nanocrystals for CO2 electroreduction reaction [5]

Methods

Results

Conclusion