Abstract
0946 09 1. Ribosomes of the blue-green alga, Anabaena flos-aquae, were isolated using a rapid lysis method and by grinding with alumina. 2. Analysis of ribosomes obtained by rapid lysis on sucrose gradients and electrophoretic gels indicated the presence of 30S, 50S, 70S, and 100S sibosomes as well as polysomes. 3. Dissociation of algal 70S and 100S ribosomes into their 30S and 50S subunits was demonstrated using a 6000xg preparation on a two-dimensional electrophoretic gel as well as a 105000xg fraction on a sucrose gradient. 4. Differences between blue-green algal and bacterial ribosomes were noted when 105000xg fractions of both were washed in ribosomal washing buffer. A major 100S or dimer peak was observed for the bacterial preparation before and after washing. The major peak in the algal preparation shifted from 100S to 70S after washing.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
