The Effects of Various Chemical Agents on Priming of Neutrophils Exposed to Weak Combined Magnetic Fields

Abstract

It has been shown that various chemical agents (ethylenediaminetetraacetic acid, zinc sulphate, ethyl alcohol, and rotenone) have different effects on preactivation (priming) of neutrophils that develop under weak combined collinear static and alternating magnetic fields (combined magnetic fields: a static field of 42 μT and an alternating field of 0.86 μT with the sum of frequencies 1.0, 4.4 and 16.5 Hz). Low concentrations (0.05%) of ethylenediaminetetraacetic acid decrease the intensity of luminol dependent chemiluminescence of neutrophils in response to an activator of the respiratory burst the peptide N-formyl-Met–Leu–Phe under the action of combined magnetic fields to a lesser extent than in the control. In contrast, ethyl alcohol (0.45%) and zinc sulphate (0.1 mM) have a greater effect on this process under the action of combined magnetic fields. Rotenone (1 μM) has a weak effect on neutrophil chemiluminescence both under the action of combined magnetic fields and in the control.