Abstract
The effects of thromboxane B 2 and 6-ketoprostaglandin F 1α on the synthesis of DNA, RNA and hexosamine-containing substances were studied. Thromboxane B 2 (1 μg/ml) added to cultures in the stationary phase caused the initiation of DNA synthesis and cell proliferation in a small proportion of cells. The same amount of thromboxane B 2 also increased [ 3H]uridine incorporation into acid-insoluble fraction by 50% in 24 h, and stimulated the production of hexosamine-containing substances 2.5 fold over the control during the first 6 h. On the other hand, 6-ketoprostaglandin F 1α was essentially inactive on these indexes except slight stimulation of DNA synthesis.
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