Abstract
Twenty-seven young adult male rats were injected intra-muscularly with 0.5 ml turpentine per day and sacrificed immediately after injection, and 1, 3, 6, 24, 48, 72, 96 and 120 hr after the start of the injections. The adrenal glands were examined histologically for evidence of stress. The submaxillary glands were stained with haematoxylin and eosin, the periodic acid-Schiff technique with 2-hour and 18-hour malt diastase digestion, the methyl green-pyronin method with ribonuclease digestion and the calcium-cobalt technique for alkaline phosphatase. During the alarm reaction the submaxillary glands showed a decrease in RNA, a decrease in the granularity of the acini, a decrease in interacinar connective tissue and an increase in granularity of the duct cells. The ducts stained more PAS-positive than the acini. A highly insoluble, heat-resistant, diastase-fast, PAS-positive material was present in the duct cells. During the stage of resistance, the duct cells showed a decrease in granules with little acinar change. No observable difference in alkaline phosphatase activity was noted.
Published Version
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