Abstract
Betula platyphylla var. japonica (Betulaceae) has been used traditionally in Asian countries for the treatment of inflammatory diseases. A recent study has reported a phenolic compound, platyphylloside from B. platyphylla, that shows inhibition on adipocyte differentiation and induces lipolysis in 3T3-L1 cells. Based on this finding, we conducted phytochemical analysis of the EtOH extract of the bark of B. platyphylla var. japonica, which resulted in the isolation of phenolic glycosides (1–4). Treatment of the isolated compounds (1–4) during adipocyte differentiation of 3T3-L1 mouse adipocytes resulted in dose-dependent inhibition of adipogenesis. In mature adipocytes, arylbutanoid glycosides (2–4) induced lipolysis related genes HSL and ATGL, whereas catechin glycoside (1) had no effect. Additionally, arylbutanoid glycosides (2–4) also induced GLUT4 and adiponectin mRNA expression, indicating improvement in insulin signaling. This suggests that the isolates from B. platyphylla var. japonica exert benefial effects in regulation of adipocyte differentiation as well as adipocyte metabolism.
Highlights
IntroductionObesity is one of the most prevalent chronic diseases worldwide
Obesity is one of the most prevalent chronic diseases worldwide1. It is a state of excessive fat accumulation in the adipose tissue which results in metabolic diseases such as insulin resistance, type 2 diabetes mellitus, and cardiovascular diseases2
Phytochemical analysis of the EtOH extract of bark of B. platyphylla var. japonica led to the isolation of phenolic glycosides including a catechin glycoside [1] and three arylbutanoid glycosides [2–4], structures of which were elucidated based on the Nuclear magnetic resonance (NMR) spectroscopic data and LC/MS analysis (Figure 1)
Summary
Obesity is one of the most prevalent chronic diseases worldwide. Obesity is one of the most prevalent chronic diseases worldwide1 It is a state of excessive fat accumulation in the adipose tissue which results in metabolic diseases such as insulin resistance, type 2 diabetes mellitus, and cardiovascular diseases. Mature adipocytes are primarily involved in the regulation of energy homeostasis by buffering lipid metabolites and secreting adipokines such as leptin and adiponectin. Adiponectin is a well-known anti-inflammatory adipokine, which regulates local adipocyte insulin sensitivity and the whole body metabolism through enhancing energy expenditure on peripheral tissues, such as liver and muscle. Dysregulation of adipokines along with inflammation in adipose tissue leads to the development of metabolic diseases such as insulin resistance
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call