The effects of osteogenic growth peptide combined with extracorporeal shock waves on osteoblast proliferation

Abstract

Objective To investigate the effects of osteogenic growth peptide (OGP) in combination with extracorporeal shock waves (ESWs) on osteoblast proliferation. Methods Passaged cells were divided into four groups for different treatments:a control group,an OGP + ESW group,an ESW group,and an OGP group.After the respective treatments,the cells were cultured for 24 h,48 h and 72 h and counted using methylthiazdy tetrazolium (MTT) and an inverted fluorescence microscope. Immunohistochemical examination was used for detecting protein kinase A (PKA) activity,and a reverse transcription-polymerase chair reaction (RT-PCR) was used for examining PKA mRNA expression at 24 and 48 hours. Results Cell counting revealed that cell proliferation in the OGP + ESW,ESW and OGP groups was significantly promoted compared with the control group.Cell proliferation was greatest in the OGP + ESW group.The immunohistochemical examination showed positive staining intensities in the OGP + ESW,ESW and OGP groups significantly higher than in the control group.The positive staining intensity in the OGP + ESW group was again the highest.PKA activity was also significantly higher in the OGP + ESW,ESW and OGP groups than in the control group with the level in the OGP + ESW group the highest. Conclusion OGP in combination with ESW has a synergistic effect in stimulating osteoblast proliferation and growth. Key words: Osteogenic growth peptide; Extroacorporeal shock waves; Protein kinase A; MC3T3-E1cells