Abstract
This chapter presents a double blind study in which human volunteers received aliquotes of keyhole limpet hemocyanin (KLH) positive, horse shoe-crab hemocyanin (HCH) positive, double positive, or double negative dialyzable leukocyte extracts (DLE) derived from individual human and bovine donors and donor pools, or aliquotes of saline. Although a non-double blind, pilot study suggested immunologically specific transfer of DTH, in three subsequent double blind experiments, only one DLE preparation transferred according to the predetermined reactivity. Thus, the results gave no statistically significant evidence for donor-related specific transfer of dermal reactivity to the test antigens. However, the results strongly suggest that human and bovine DLEs augmented a low-level reactivity observed in the recipient population. Apart from one preparation, all DLEs of various immunological specificities significantly augmented KLH and HCH skin reactivities. These skin reactivities did not correlate with in vitro lymphocyte transformation or lymphokine-secretion assays. The nonactive DLE (Pool II) may have been inactivated in the process of preparing it.
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