Abstract
This study investigated the influence of different titanium surfaces on the differentiation of rat osteoblast-like cells (osteo-1). Osteo-1 cells were cultured on the following titanium surfaces: 1) pretreated, smooth surface (PT); 2) sandblasted and acid etched surface (SLA); and 3) sandblasted and acid-etched surface rinsed under nitrogen protection to prevent exposure to air and preserved in isotonic saline solution (modSLA). Cell metabolism, total protein content, collagen content and alkaline phosphatase (AP) activity and the formation of calcified nodules were analyzed. The titanium surface did not influence cell metabolism, total protein content and collagen content. The SLA surface influenced cell differentiation, with the observation of a significant reduction of AP activity and formation of calcified nodules after 21 days compared to the PT surface. No difference was observed between the PT and modSLA surfaces. All titanium surfaces tested permitted the full expression of the osteoblast phenotype by osteo-1 cells, including matrix mineralization.
Highlights
Various in vitro studies have shown that the surface roughness of titanium reduces the proliferation of osteoblast-like cells and increases cell differentiation by increasing alkaline phosphatase (AP) activity, protein synthesis, osteocalcin production and the formation of bone-like nodules [1]-[3]
Cell metabolism was not affected by the titanium surface topography (p = 0.6407) (Figure 2)
AP activity and the formation of calcified nodules after 21 days of culture were higher in the PT group compared to the sandblasted and acid etched (SLA) group, no difference was observed between the PT and modSLA groups
Summary
Various in vitro studies have shown that the surface roughness of titanium reduces the proliferation of osteoblast-like cells and increases cell differentiation by increasing alkaline phosphatase (AP) activity, protein synthesis, osteocalcin production and the formation of bone-like nodules [1]-[3]. How to cite this paper: Cirano, F.R., Togashi, A.Y., Marques, M.M., Pustiglioni, F.E. and Lima, L.A.P.A. (2015) The Effects of Different Titanium Surfaces on the Behaviour of Osteoblast-Like Cells. The biological activity of TiO2 influences protein adsorption to the titanium surface [6]
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