The effect ofin vivo stimulation on the cytology of neuromuscular junctions of locust flight muscles

Abstract

The ultrastructure of neuromuscular junctions in dorsoventral (tergosternal) flight muscle no. 113 and dorsolongitudinal flight muscle no. 112 of the locust Schistocerca gregaria is described. Following in vivo stimulation by enforced flight, morphological and statistical analyses reveal cytological changes at these junctions suggestive of vesicular release of neurotransmitter and membrane recycling. Flight periods from 30 min to 3 h produced a progressive decrease in the density of terminal synaptic vesicles, an increase in terminal surface area and circumference, increases in the occurrence of membranous cisternae, increases in mitochondrial numbers, and increases in the frequency of coated pits.