THE EFFECT OF VACCINIUM MYRTILLUS AND VACCINUM VITIS-IDAEA FRUITS EXTRACTS ON THE ANTIOXIDANT SYSTEM OF MAMALIAN ORGANISMS

Abstract

SUMMARY The present paper deals with the study of some Vaccinium myrtillus and Vaccinum vitis-idaea fruits extracts effects on the antioxidant system of guinea pigs. Both extracts stimulated the antioxidant system of the cells: it doesnt not effect the normal function of the liver and stimulates the absorption of calcium and phosphorus. Especially; Vaccinum myrtil us stimulates the haemoglobine synthesis and also the iron absorption. It is a much more antioxidant as Vaccinum vitis-idaea as there were registered lower levels of malondial dehide (MDA) respectively higher concentration of reduced glutathione (GSH) in comparison with the Vaccinum vitis-idaea . In the same time lower activities of superoxid di smutase (SOD); glutathione peroxidase (G-px) and glutathione reduc tase (G-red) were registered in the treatment with Vaccinum myrtilus extract. Free radicals; respectively reactive species of t he oxigen (ROS) in physiological concentration are stimulating the development and t he cell division. A mutation at cell level can be the cause of permanent deviation of the oxid ative reactions with RSO development in excess. In very large doses; ROS lead to apoptosis. As there are quite few studies in this field; in the present work we study the antioxidant proper ties of some Vaccinium myrtillus and Vaccinum vitis-idaea fruits extracts on the antioxidant system of guine a pigs; as a possible oxidative stress barrier as both of the extracts co ntain antioxidant components as flavonoides; antocianines; etc. The experiment was carried on 36 adult female guinea pigs (divided in three groups) and maintained in good physiological conditions. Th ey were divided in three groups. Each group included 12 guinea pigs.L1- control; received tap water ad libitum during 14 days; L2 - received during 14 days 0.5 ml i.m. of the Vaccinium myrtillus extrac t; L3- received during 14 days 0.5 ml i.m. of the Vaccinium vitis-idaea extract After 14 days blood (on heparine); by cardiac punction and tissue samples were collect ed under general narcosis from L1; L2 and L3. The data are presented as means ± S.D. values. ANOVA; TTest were used. The best antioxidant properties has the Vaccinium myrtillus extract as it maintained a normal function of the liver (GPT; GOT and GGT were situated in normal values) and stimulates the absorption of calcium and phosphorus ; the haemoglobine synthesis and also the