Abstract
Leaves of wild Vaccinium spp. berries are considered a valuable source of antioxidants, mainly belonging to polyphenols, specifically flavonoids provide significant protection against the development and progression of many chronic pathological conditions including cancer, diabetes, osteoporosis, cardio-vascular and neurodegenerative diseases. Targeted high-performance liquid chromatography-electro spray ionization-high resolution mass spectrometry operated in the tandem mode (LC-ESI/HRMS2) approach was applied to evaluate and compare the metabolite profiles of bilberry (Vaccinium myrtillus L.) and lingonberry (Vaccinium vitis-idaea L.) leaves originating from the Rhodope Mountain in Bulgaria during growing season. In total, 55 different phenolic compounds, 52 in bilberry and 48 in lingonberry were identified and quantified, including flavan-3-ols, proanthocyanidins, flavonols and their glycosides, iridoids, cinchonain isomers, various phenolic acid conjugates. The contents of different metabolites varied remarkably in bilberry leaves, whereas in lingonberry leaves the metabolites maintained quite constant levels during the growing season.
Published Version
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