The effect of the nanoscale form of 20-hydroxyecdysone on the antioxidant status and natural resistance of the rabbit organism

Abstract

Current trends in the development of new drugs are based on the creation of nanostructured compositions with improved characteristics. One of the approaches to creating a nanoscale form of drugs, in particular 20-hydroxyecdysone, is the synthesis of its clathrate complex with the clathrate-forming arabinogalactan. Thus obtained nanoscale form of 20-hydroxyecdysone provides an improvement in its physico-chemical properties, which leads to increased absorption, increased bioavailability and, ultimately, to reduce therapeutic doses. In the present work, the effect of the created nanoscale form of 20-hydroxyecdysone on the function of the antioxidant defense system, natural resistance, growth and development of Sovetskaya Chinchilla rabbits was studied for the first time. It has been established that the nanoscale form of 20-hydroxyecdysone, when administered to rabbits at doses of 1.0 and 5.0 mg / kg body weight, contributes to a statistically significant increase in serum BASK and LASK levels, increased activity of the enzymatic unit of the antioxidant system, in particular SOD and catalase in erythrocyte lysate. On the contrary, the content of MDA in the blood plasma was statistically significant [P≤0.05 — P≤0.02] was lower in rabbits of the experimental groups compared with the control, which may indicate a decrease in lipid peroxidation processes in animals under the influence of nanoscale form 20-hydroxyecdysone. In the rabbits of the experimental groups, compared with the control, they were statistically significantly higher by 32-38% [P≤0.05–P≤0.01] absolute and average daily weight gain of rabbits. The optimal dose of the nanoscale form of 20-hydroxyecdysone is dose 1, 0 mg / kg rabbit body weight.