The effect of the imbalance of the neurotransmitter systems of the dorsal hippocampus on the activity of alveolar macrophages and lung surfactant

Abstract

The purpose of the study. The paper analyzes the metabolism of surfactant lipids and phagocytic activity of alveolar macrophages with an imbalance of the neurotransmitter systems of the dorsal hippocampus. Materials and methods. Male rats were microinjected with L-glutamate and GABA into the dorsal hippocampus bilaterally by stereotactic coordinates. Studies included determination of surfactant lipid fractions (thin-layer chromatography method), total phospholipids and their surface-active properties (Wilhelmi method), phospholipase activity, evaluation of endopulmonary cytogram, phagocytic index, and phagocytic number. Results. It was found that the introduction of L-glutamate was accompanied by an increase in phospholipids in the surfactant due to «inert» fractions of phosphatidylserine, phosphatidylinositol, and a low-active fraction of phosphatidylethanolamine against the background of a decrease in the intensity of phospholipase hydrolysis and the ability of macrophages to phagocytosis. The introduction of GABA led to redistribution of phospholipid fractions toward a decrease in phosphatidylcholine surfactant and an increase in the proportion of lysophosphatidylcholine under conditions of phospholipase activation and macrophage phagocytic activity. Conclusions The imbalance of the glutamate and GABAergic systems of the hippocampus has a multidirectional effect on the composition of surfactant lipids, the ability of alveolar macrophages to phagocytosis, and is characterized by the deterioration of the surface-active properties of the lungs.