The effect of single acute cocaine exposure on the local network activity of PFC neurones in mice in vivo as revealed by optogenetic methods

Abstract

Event Abstract Back to Event The effect of single acute cocaine exposure on the local network activity of PFC neurones in mice in vivo as revealed by optogenetic methods T. Tompa1* and A. Lavin1 1 Med. Univ. of South Carolina, Department of Neuroscience, United States Drugs of abuse modify cortical circuitry on the short and long term and thereby lead to catastrophic effects on individual and social level. Cocaine modifies prefrontal physiology in still not known ways. We use optogenetic methods to reveal local effects. We induced expression of channelorodopsin-2 in PV fast-spiking interneurons with AAV virus injection in PV-Cre knock-in mice. Upon expression of the protein we could identify and stimulate PV neurons with a 470 nm blue laser. Using different frequency laser stimulation (1 Hz, 40 Hz) we performed single-unit, multiunit and field-recordings in the mPFC of mice in vivo. We measured the firing frequency of the neurons, latency, amplitude and the frequency-power-distribution of the field responses. Upon a single acute IP exposure of cocaine ((15mg/kg)) we detected in the mPFC a: 1) a slight shortening of the inhibitory effect on the local network 2) diminishing of the variability/variance of the evoked LFP responses 3) earlier peak of the postinhibitory rebound activity and 4) changes in the frequency power spectrum of the responses. Our preliminary results suggest that a single exposure to cocaine causes characteristic changes in cortical network activity. These changes may underlie the behavioral effects of cocaine, even after a single exposure and thereby yield long-term neuroadaptation. Keywords: Behaviour and cognition, Neuroscience Conference: 13th Conference of the Hungarian Neuroscience Society (MITT), Budapest, Hungary, 20 Jan - 22 Jan, 2011. Presentation Type: Abstract Topic: Behaviour and cognition Citation: Tompa T and Lavin A (2011). The effect of single acute cocaine exposure on the local network activity of PFC neurones in mice in vivo as revealed by optogenetic methods. Front. Neurosci. Conference Abstract: 13th Conference of the Hungarian Neuroscience Society (MITT). doi: 10.3389/conf.fnins.2011.84.00056 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 03 Mar 2011; Published Online: 23 Mar 2011. * Correspondence: Dr. T. Tompa, Med. Univ. of South Carolina, Department of Neuroscience, Charleston, United States, tompa@musc.edu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers T. Tompa A. Lavin Google T. Tompa A. Lavin Google Scholar T. Tompa A. Lavin PubMed T. Tompa A. Lavin Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.