The effect of sepsis serum on intercellar adhesion molecule-1 of rat intestinal mucosa microvascular endothelial cells and the intervention of Adrenomedullin

Abstract

：Objective To observe thelevels of intercellar adhesion molecule-1(ICAM-1)of rat intestinal mucosa microvascularendothelial cells in sepsis rat,and to investigate the possible protective effect ofadrenomedullin on the expression of intercellar adhesion molecule-1(ICAM-1)of ratintestinal mucosa microvascular endothelial cells,and to confirm whether adrenomedullincan decrease released inflammatory factors to slow down or inhibit the occurrence anddevelopment of sepsis.Methods The rat intestinal mucosa microvascular endothelial cellswere isolated and cultured in vitro,and it was divided into sham operated(sham operatedserum),sepsis serum(with addition of sepsis serum)and ADM serum(with 0.lng/kgadrenomedullin 6h after sepsis treatment)groups.The soluble ICAM-1(sICAM-1)content inRIMMVECs culture supernatant was measured by ELISA at the 24th hour after serumstimulation.Results The ICAM-1 content in the supernatant of sepsis group [(0.33 ±0.04)ng/L]was obviously higher than that in sham operated [(0.15 ± 0.02)ng/L] and ADM [(0.17 ±0.04)ng/L] group(P ＜ 0.05),but ADM group was nosignificantly different compared with sham operated group(P ＞0.05).Conclusions ADM could decrease theexpression of ICAM-I in RIMMVECs,and the low level of ICAM-1 could suppress its downstreaminflammation factors and inhibit the occurrence and development of sepsis. Key words: Sepsis/BL/ME/DT; Intercellular adhesion molecule-1/ME; Endothelium,vascular/ME; Adrenomedullin/PD