Effects of growth arrest-specific protein 6 on blood coagulation in septic mice

Abstract

Objective To investigate the effects of growth arrest-specific protein 6 on blood coagulation in septic mice. Methods Septic mice model was induced by cecal ligation and puncture (CLP). One hundred and twenty male BALB/c mice of clean grade were randomly (random number) divided into six groups: sham operation group (n=20) , CLP group (n=20) , CLP +vehicle group (n=20) , CLP +Gas6 (Growth Arrest-Specific Protein 6) (1 μg) group (n=20) , CLP +Gas6 (3 μg) group (n =20) and CLP +Gas6 (6 μg) group (n=20). All the survived mice were sacrificed after 24 h of operation. Plasma prothrombin time (PT) , thrombin time (TT) , active partial thrombo-plastin time (APTT) , fibrinogen (Fbg) , d-dimer (DD) and Platelets (PLT) count was measured. Plasma tissue factor (TF) , P-selectin, Intercellular Adhesion Molecule 1 (ICAM-1) was measured by ELISA method. Statistical analyses were performed using one-way analysis of variance (SPSS18.0, Chicago, USA) test followed by Bonferroni test to compare three or more groups or by Student' s t-test to compare two groups. Results Plasma PT, TT, APTT, DD, TF, P-selectin and ICAM-1 in CLP group was longer or higher than that in sham group (P=0.000) , while Fbg and PLT in CLP +vehicle group were lower than that in sham group (P=0.000). Treatment with rmGas6 significantly increase plasma PT, TT, APTT, Fbg, DD, PLT, TF, P-selectin and ICAM-1 in septic mice (P <0.01). Conclusions Gas6 can activate coagulation system and aggravate coagulation dysfunction in septic mices, which may partially through activate endothelial cells and up-regulate TF levels. Key words: Growth arrest-specific protein 6; Sepsis; Blood coagulation; Endothelial Cells; Blood Platelets; Tissue factor; P-selectin; Intercellular Adhesion Molecule 1